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Biomonitoring
(Whole Effluent Toxicity (WET)) was promulgated in 60 FR
53529, October 16, 1995.
Whole
effluent toxicity (WET) test methods are used by the EPA
to protect aquatic life under NPDES data gathering and compliance
monitoring. The agency approved WET methods are specified
in 40 CFR 136.3, Table IA. American Interplex Corporation
analytical services offered for Biomonitoring are inclusive
of methodology cited in EPA-821-R-02-012 "Methods for
Measuring the Acute Toxicity of Effluents and Receiving
Waters to Freshwater and Marine Organisms" and EPA-821-R-02-013
"Short-Term Methods for Estimating the Chronic Toxicity
of Effluents and Receiving Water to Freshwater Organisms".
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The Acute toxicity tests generally involve exposure of test organisms
to each of five effluent concentrations and a control. The test
duration ranges from 24-48 hours.
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| 24-Hr Acute Toxicity Test, Ceriodaphnia dubia |
EPA 2002 |
| 24-Hr Acute Toxicity Test, Daphnia pulex |
EPA 2021 |
| 24-Hr Acute Toxicity Test, Pimephales promelas |
EPA 2000 |
| 48-Hr Acute Toxicity Test, Ceriodaphnia dubia |
EPA 2002 |
| 48-Hr Acute Toxicity Test, Daphnia pulex |
EPA 2021 |
| 48-Hr Acute Toxicity Test, Pimephales promelas |
EPA 2000 |
The Chronic tests are seven-day exposure for estimating the chronic toxicity of effluents and receiving waters to the fathead minnow, Pimephales promelas, and the cladoceran, Ceriodahpnia dubia.
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| 7-Day Static Renewal Survival and Reproduction Test, Ceriodaphnia dubia |
EPA 1000 |
| 7-Day Static Renewal Survival and Growth Test,
Larval Fathead Minnow, Pimephales promelas |
EPA 1002 |
